Hygiena 2013, 58(2):75-78 | DOI: 10.21101/hygiena.a0968

Issues of Presence of Microorganisms on Non-sterile Examination Gloves

Petra Telekesová1, Ladislav Dzan2
1 Krajská nemocnice Liberec a. s., Oddělení klinické mikrobiologie a imunologie, Liberec
2 Krajská nemocnice Liberec a. s., Oddělení ústní, čelistní a obličejové chirurgie, Liberec

Preface and Task Objective: With respect to economic aspects of providing medical care, the issue of using sterile versus nonsterile "examination" gloves in operations verging on invasivity and in diagnostic procedures has been gaining increasing attention. This article's objective is evaluation of presence of bacterial microorganisms on the surface of nonsterile examination gloves in quantities that allow valid statistical result assessment. Two hypotheses were proposed: H0: disposable nonsterile gloves may not be considered safe for increased risk of nosocomial infection in examinations or minor surgeries in immunocompetent patients. H1: disposable nonsterile gloves may be considered safe from the point of view of possible risk of nosocomial infection in examinations or minor surgeries in immunocompetent patients. Materials and Methods Used: The microbiological surface smears of 3,000 talc-free nonsterile examination gloves were tested using common cultivation method - inoculation on culture media. Results, Arguments and Conclusions: A conventional statistical hypothesis test method on the binomial parameter distribution against a monomial alternative was applied to process the results for obligatory pathogenic microorganisms. Statistical evaluation of the results from primary cultivation microbiological examination allows to reject hypothesis H0 and validate hypothesis H1. On the other hand, the finding of obligatory pathogenic microorganism of methicillin-sensitive staphylococcus aureus grown through inoculation in a single sample of all glove samples does not any longer allow to reject hypothesis H0.

Keywords: non-sterile examination gloves, epidemiological study, microorganisms - occurence

Received: October 2012; Accepted: January 24, 2013; Published: June 1, 2013  Show citation

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Telekesová P, Dzan L. Issues of Presence of Microorganisms on Non-sterile Examination Gloves. Hygiena. 2013;58(2):75-78. doi: 10.21101/hygiena.a0968.
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