Hygiena 2013, 58(2):70-74 | DOI: 10.21101/hygiena.a0967

Comparison of the Excretion Rate of Selected Biomarkers of Benzene Exposure

Ivica Bajusová1, Peter Kolarčik2, Michal Ihnatko3, Tatiana Kimáková2, Ľubomír Legáth1
1 Klinika pracovného lekárstva a klinickej toxikológie LF Univerzity P. J. Šafárika a Univerzitnej nemocnice L. Pasteura, Košice
2 Univerzita P. J. Šafárika v Košiciach, LF, Ústav verejného zdravotníctva, Košice
3 Nemocnica Košice-Šaca, 1. súkromná nemocnica, Oddelenie pracovného lekárstva a klinickej toxikológie, Košice

Benzene has hematoxic effects, causes damage to the central nervous system and immune system, is classified as a Category 1 carcinogen, and a Category 2 mutagen. Benzene exposure is limited by public health legislation. A total of 99 biological samples of benzene-exposed workers and 19 biological samples of non-exposed patients in a control group were examined. Concentration determination of two selected biomarkers of benzene exposure was performed using two different analytical methods. Trans,transmuconic acid was determined by chromatography and phenol was determined by spectrophotometry. The purpose of the study was to compare their excretion rate in both monitored groups, and to prove appropriateness of chromatographic determination of trans,trans-muconic acid in urine for quick and reliable biomonitoring of the working environment. The average phenol concentration in both groups varied at physiological levels: mean (SD) was 11.07 (8.51) mg.g-1 creatinine for a benzene-exposed group, and 12.16 (11.42) mg.g-1 creatinine for a control group. As for trans,trans-muconic acid, the average concentration was 0.64 (0.95) mg.g-1 creatinine in case of benzene-exposed workers compared to 0.29 (0.24) mg.g-1 creatinine for a control group. Concentration of trans,trans-muconic acid in urine from exposed workers did not exceed a recommended concentration limit for Threshold Limit Value level for benzene, but exceeded a recommended Biological Exposure Indices value set by the American Conference of Governmental Industrial Hygienists. A statistic t-test revealed a significant difference in measured values of excreted trans,transmuconic acid between a control group of patients and benzene-exposed workers proving appropriateness of determination for quick and reliable bio-monitoring of the working environment.

Keywords: benzene exposure, biomarkers, working environment

Received: July 2012; Accepted: December 10, 2012; Published: June 1, 2013  Show citation

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Bajusová I, Kolarčik P, Ihnatko M, Kimáková T, Legáth Ľ. Comparison of the Excretion Rate of Selected Biomarkers of Benzene Exposure. Hygiena. 2013;58(2):70-74. doi: 10.21101/hygiena.a0967.
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