Hygiena 2009, 54(3):99-103

Cytogenetic Methods and Occupational Health Protection - a Laboratory Worker's Experience

Jaromíra Kůsová
Zdravotní ústav se sídlem v Ostravě

Cytogenetic methods including cytogenetic analysis of chromosomal aberrations of peripheral lymphocytes have been using in monitoring of largely occupational exposure to genotoxic factors in Czech Republic and other countries for many years. In view of the fact that problems of genetic toxicology are quite remote for the health protection experts, the article submits some scientific knowledge from the discipline of genetic toxicology and selected experience and outputs in context with using of the "conventional cytogenetic analysis of chromosomal aberrations in human lymphocytes" in connection with the health safety of people, working at Moravskoslezsky region, professionally exposed to mutagens and carcinogens. Biological identification of the resulting genotoxic effect could develop more effective beneficial intervention into this safety.

Keywords: genetic toxicology, chromosomal aberrations, occupational health protection

Received: December 2008; Accepted: June 5, 2009; Published: September 1, 2009  Show citation

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Kůsová J. Cytogenetic Methods and Occupational Health Protection - a Laboratory Worker's Experience. Hygiena. 2009;54(3):99-103.
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    References

    1. Albertini RJ, Anderson D, Douglas GR, Hagmar L, Hemminki K, et al. IPCS guidelines for the monitoring of genotoxic effects of carcinogens in human. Mutat Res. 2000 Aug;463(2):111-72. Go to original source...
    2. Bonassi S, Norpa H, Ceppi M, Strömberg U, Vermeulen R, et al. Chromosomal abberation frequency in lymphocytes predicts the risk of cancer. Results from a pooled kohort study of 22 358 subjects in 11 countries. Carcinogenesis. 2008 Jun;29(6):1178-83. Go to original source... Go to PubMed...
    3. Sarrif A, Wiegand HJ, Knudsen LE, Kyrtopoulos SA, Srám RJ, Vrijhof H. Editorial. Biomarkers in children and adults - introduction and overview. Toxicol Lett. 2007 Jul 30;172 (1-2):1-3. Go to original source... Go to PubMed...
    4. Fučić A, Znaor A, Strnad M, van der Hel O, Aleksandrov A, Miskov S, et al. Chromosome damage and cancer risk in the workplace: The example of cytogenetic surveillance in Croatia. Toxicol Lett. 2007 Jul 30;172(1-2):4-11. Go to original source... Go to PubMed...
    5. Garry S, Nesslany F, Aliouat E, Haguenoer JM, Marzin D. Hematite (Fe2O3) enhances benzo[a]pyrene genotoxicity in endotracheally treated rat, as determined by Comet Assay. Mutat Res. 2003 Jul 8;538(1-2):19-29. Go to original source...
    6. Hagmar L, Strömberg U, Bonassi S, Hansteen IL, Knudsen LE, Lindholm C, et al. Impact of types of lymphocyte chromosomal aberrations on human cancer risk: results from Nordic and Italic cohorts. Cancer Res. 2004 Mar 15;64(6):2258-63. Go to original source... Go to PubMed...
    7. Kučera I, Hlaváč P. Kategorizace prací ve vazbě na profesionální onemocnění hlášená v r. 2007. Čes Prac Lék. 2008;9 (2-3):81-6.
    8. Kůsová J. Proč využívat cytogenetickou analýzu periferních lymfocytů jako test expozice a časných biologických účinků genotoxických látek v pracovním prostředí. Čes Prac Lék. 2007;8(3):142-5.
    9. Metody biologického monitorování genotoxických účinků faktorů prostředí - standardní metodika. AHEM. 1989;Příl. 20:1-56.
    10. Merlo DF, Ceppi M, Stagi E, Bocchini V, Sram RJ, Rössner P. Baseline chromosome aberrations in children. Toxicol Lett. 2007 Jul 30;172(1-2):60-7. Go to original source... Go to PubMed...
    11. Norppa H, Bonassi S, Hansteen IL, Hagmar L, Strömberg U, Rössner P, et al. Chromosomal aberrations and SCEs as a biomarker of cancer risk. Mutat Res. 2006 Aug 30;600 (1-2):37-45. Go to original source... Go to PubMed...
    12. Očadlíková D, Bavorová H, Šmíd J. Cytogenetická analýza periferních lymfocytů. Aktualizace platné standardní metodiky. AHEM [online]. 2007 [cit. 2008-10-22];(1). Dostupný z: http://www.szu.cz/uploads/documents/knihovna_SVI/pdf/2007/full_2007_01.pdf.
    13. Státní zdravotní ústav [online]. REGEX - závěrečná zpráva 2006 [cit. 2008-10-22]. Dostupný z: http://www.szu.cz/uploads/documents/cpl/REGEX/zaver_zprava_06.pdf
    14. Rössner P, Bavorová H, Očadlíková D. Metody biologického monitorování genotoxických účinků faktorů prostředí: cytogenetická analýza periferních lymfocytů. AHEM. 2000; 5:1-33.
    15. Rössner P, Boffeta P, Ceppi M, Bonassi S, Smerhovsky Z, Landa K, et al. Chromosomal aberrations in lymphocytes of healthy subjects and risk of cancer. Environ Health Perspect. 2005 May;113(5):517-20. Go to original source... Go to PubMed...
    16. Rössner P, Černá M, Bavorová H, Pastorková A, Očadlíková D. Monitoring of human exposure to occupational genotoxicants. Cent Eur J Public Health. 1995 Nov;3(4):219-23.
    17. Rössner P, Šrám RJ, Bavorová H, Očadlíková D, Černá M, Švandová E. Spontaneous level of chromosomal aberrations in peripheral blood lymphocytes of control individuals of the Czech Republic population. Toxicol Lett. 1998 Aug; 96-97:137-42. Go to original source... Go to PubMed...
    18. Szendi K, Murányi E, Gerencser G, Varga C. Biomarkers studies in ionizing radiation and cytostatic exposure. Cent Eur J Public Health. 2007 Nov;15 Suppl:S17.
    19. Šmerhovský Z, Landa K, Rössner P, Brabec M, Zudova Z, Hola N, et al. Risk of cancer in an occupationally-exposed cohort with increased level of chromosomal aberrations. Environ Health Perspect. 2001 Jan;109(1):41-5. Go to original source... Go to PubMed...
    20. Šrám RJ, Rössner P, Šmerhovský Z. Cytogenetic analysis and occupational health in the Czech Republic. Mutat Res. 2004 Jan;566(1):21-48. Go to original source...
    21. Sram RJ, Rössner P. Cytogenetic analysis and occupational health. In: Obe G, Vijayalaxmi, editors. Chromosomal alterations: methods, results and importance in human health. Berlin-Heidelberg: Springer Verlag; 2007. p. 323-37.
    22. Sram RJ, Rössner P, Beskid O, Bavorova H, Ocadlikova D, Solansky I, et al. Chromosomal aberration frequencies determined by conventional methods: Paralel increases over time in the region of petrochemical industry and througout the Czech Republic. Chem Biol Interact. 2007 Mar 20;166 (1-3):239-44. Go to original source... Go to PubMed...

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